Bio Hydrogen

Work area

Biofuel Preparation OBeG is continuously attempting to optimize the techniques for the production of biofuels.
This area covers the bioalchohols, biohydrogen and biomethane.
Our group is investigating all aspects of biofuels production from pretreatment of the biomass, microbial degradation of the biomass to yield enhancement.


To produce bio-hydrogen from a digestion process instead of methane, the operating conditions have to be changed in such a manner that the methane producing bacteria are inhibited.This is known as dark fermentation. The common approach to produce bio-hydrogen with a digester is to change the pH of the system to values below 6.5 or above 8. During the pre-treatment stage, heat is supplied to kill the methane producing bacteria. Typical hydrogen yields range from 1 to 2 mol of hydrogen per mol of glucose, which results in 80-90% of the initial organic compounds remaining in the wastewater in the form of various volatile fatty acids (VFAs).

OBeG’s core know-how is based around the dark fermentation process where water-to-oxygen (H2O/O2) ratio is added to the digestion process to produce more hydrogen than the conventional system. So far, OBeG can demonstrate in their experiments a hydrogen yield of 6 mol per mol of glucose when using a water-to-oxygen ratio of 2. To slow down the methanogenesis OBeG has developed proprietary process know-how. For the initiation phase of the digestion process the addition of heavy metals salts slows down the methanogenesis and increases the hydrogen formation rates at the same time. The hydrogen produced can be supplied to a biogas fermenter to improve the quality and yield of methane as well.